Effect of thyroid hormone on the expression of mRNA encoding sarcoplasmic reticulum proteins.

Author:

Arai M1,Otsu K1,MacLennan D H1,Alpert N R1,Periasamy M1

Affiliation:

1. Department of Physiology and Biophysics, University of Vermont College of Medicine, Burlington 05405.

Abstract

The purpose of this study was to determine the expression of genes encoding various sarcoplasmic reticulum components that are functionally coupled with calcium release, uptake, and storage function during cardiac hypertrophy induced by thyroid hormone. Hyperthyroidism was induced in two groups of rabbits by the injection of 200 micrograms/kg L-thyroxine (T4) daily for 4 days (T4-4-day group) and 8 days (T4-8-day group). Hypothyroidism was induced in another group of rabbits by adding 0.8 mg/ml propylthiouracil to the drinking water for 4 weeks. The relative expression level of mRNA encoding different sarcoplasmic reticulum proteins was determined by RNA slot blot and Northern blot analysis. In hyperthyroid hearts, the steady-state level of cardiac ryanodine receptor mRNA and sarcoplasmic reticulum cardiac/slow-twitch Ca(2+)-ATPase mRNA were both increased to 147% (T4-4-day group) and 186% (T4-8-day group) of control, respectively, but decreased to 71% and 75%, respectively, in hypothyroid ventricles. The mRNA level for phospholamban was decreased in both hyperthyroidism (T4-8-day group, 72%) and hypothyroidism (77%) in these hearts. On the other hand, calsequestrin mRNA levels did not change in hyperthyroid and hypothyroid ventricles. In accord with the changes in Ca(2+)-ATPase mRNA levels, the Ca(2+)-ATPase protein was increased to 199% (T4-8-day group) in hyperthyroid ventricles and decreased to 86% of control in hypothyroid ventricles. The expression levels of ryanodine receptor, Ca(2+)-ATPase, phospholamban, and calsequestrin mRNAs were similarly altered in skeletal muscle tissues from hyperthyroid and hypothyroid rabbits. These results indicate that the mRNA levels of sarcoplasmic reticulum proteins responsible for calcium release and calcium uptake are coordinately regulated in response to changes in thyroid hormone level in both heart and skeletal muscle. These changes in mRNA level should lead to changes in protein levels and thus to altered calcium release and uptake in the chronic stages of hyperthyroidism and hypothyroidism.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Cardiology and Cardiovascular Medicine,Physiology

Reference57 articles.

1. Scheuer J: Alteration in sarcoplasmic reticulum in cardiac hypertrophy in Tarazi RC Dunbar JB (eds): Perspectives in Cardiovascular Research. New York Raven Press Publishers 1983 vol 8 pp 111-122

2. Hamrell BB Alpert NR: Cellular basis of the mechanical properties of hypertrophied myocardium in Fozzard HA Haber E Jennings RB Katz AM Morgan HE (eds): The Heart and Cardiovascular System. New York Raven Press Publishers 1986 pp 1507-1524

3. Influence of hyperthyroidism on glycerol-extracted cardiac muscle from rabbits

4. Determinants of energy utilization in the activated myocardium;Alpert NR;Fed Proc,1986

5. The effect of hyperthyroidism on the sarcoplasmic reticulum and myosin ATPase of dog hearts

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