Selective Defect in Nitric Oxide Synthesis May Explain the Impaired Endothelium-Dependent Vasodilation in Patients With Essential Hypertension

Abstract

Background —Patients with essential hypertension have impaired endothelial NO activity, but the mechanism underlying this abnormality is unknown. Methods and Results —To investigate whether the endothelial dysfunction of hypertensive patients is related to a selective defect in NO synthesis, we studied the forearm blood flow responses to intra-arterial infusion of acetylcholine (7.5 to 30 μg/min), an endothelial agonist linked to NO synthase through the Ca 2+ signaling pathway, and isoproterenol (50 to 200 ng/min), a β-adrenoceptor agonist that stimulates NO production by increasing intracellular cAMP, in 12 normotensive subjects and 12 hypertensive patients. The infusion of isoproterenol was repeated during the concurrent blockade of NO synthesis by N G -monomethyl- l -arginine (L-NMMA; 4 μmol/min). The vasodilator response to acetylcholine was significantly reduced in hypertensives compared with normotensives (maximum blood flow: 10.4±4.6 versus 14.4±3.7 mL · min −1 · dL −1 ; P =.008). However, the vasodilator effect of isoproterenol was similar in normotensives and hypertensives (maximum blood flow: 14.4±5.4 versus 13.5±5 mL · min −1 · dL −1 ; P =.56) and was significantly (both P <.01) and equally blunted by L-NMMA in both groups (maximum blood flow: 11±3 mL · min −1 · dL −1 in normotensives versus 10.8±3.9 mL · min −1 · dL −1 in hypertensives; P =.77). The vasodilator response to sodium nitroprusside (0.8 to 3.2 μg/min), an exogenous NO donor, was similar in both groups and was not modified by L-NMMA. Conclusions —Hypertensive patients have impaired endothelium-dependent vasodilation in response to acetylcholine but preserved NO activity in response to β-adrenergic stimulation. These findings suggest that the endothelial dysfunction in essential hypertension is due to a selective abnormality of NO synthesis, probably related to a defect in the phosphatidylinositol/Ca 2+ signaling pathway.