Myocardial Fibrosis and Stiffness With Hypertrophy and Heart Failure in the Spontaneously Hypertensive Rat

Abstract

Background Fibrosis is commonly found in association with cardiac hypertrophy and failure, but the relation of the connective tissue response to the development of impaired cardiac function remains unclear. We examined passive myocardial stiffness, active contractile function, and fibrosis in the spontaneously hypertensive rat (SHR), a model of chronic pressure overload in which impaired cardiac function follows a long period of stable hypertrophy. Methods and Results We studied the passive and active mechanical properties of left ventricular (LV) papillary muscles isolated from normotensive Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR) at the ages of 12 months and 20 to 23 months. Seven of 15 SHR between 20 and 23 months of age had findings consistent with heart failure (SHR-F). In comparison to preparations from WKY rats and nonfailing SHR (SHR-NF), papillary muscles from the SHR-F group demonstrated increased passive stiffness (central segment exponential stiffness constant, k cs : SHR-F 95.6±19.8, SHR-NF 42.1±9.7, WKY rats 39.5±9.5 (mean±SD); SHR-F P <.01 versus SHR-NF, WKY rats). The increase in stiffness was associated with an increase in LV collagen concentration (SHR-F 8.71±3.14, SHR-NF 5.83±1.20, WKY rats 4.78±0.70 mg hydroxyproline/g dry LV wt; SHR-F P <.01 versus SHR-NF, WKY rats); an increase in interstitial fibrosis, as determined histologically (SHR-F 13.5±8.0%, SHR-NF 4.9±2.1%, WKY rats 3.6±0.8%; SHR-F P <.01 versus SHR-NF, WKY rats); and impaired tension development (SHR-F 3.18±1.27, SHR-NF 4.41±1.04, WKY rats 4.64±0.85 kdyne/mm 2 ; SHR-F P <.05 versus SHR-NF; P <.01 versus WKY rats). Conclusions The development of heart failure in the aging SHR is associated with marked myocardial fibrosis, increased passive stiffness, and impaired contractile function relative to age-matched nonfailing SHR and nonhypertensive control animals. These data suggest that fibrosis or events underlying the connective tissue response are important in the transition from compensated hypertrophy to failure in the SHR.