Cross-Reactivity of Anti-CagA Antibodies With Vascular Wall Antigens

Author:

Franceschi Francesco1,Sepulveda Antonia R.1,Gasbarrini Antonio1,Pola Paolo1,Silveri Nicolò Gentiloni1,Gasbarrini Giovanni1,Graham David Y.1,Genta Robert M.1

Affiliation:

1. From the Departments of Pathology (F.F., R.M.G.) and Medicine (D.Y.G., R.M.G.), VA Medical Center and Baylor College of Medicine, Houston, Tex; Department of Pathology (A.R.S.), University of Pittsburgh, Pittsburgh, Pa; and Department of Internal Medicine (F.F., A.G., P.P., N.G.S., G.G.), Catholic University of Rome, Italy.

Abstract

Background Helicobacter pylori -CagA positive strains have been shown to be associated with atherosclerosis. However, the pathogenesis is still undetermined. The aim of this study was to determine whether anti-CagA antibodies cross-react with antigens of normal and atherosclerotic arteries. Methods and Results Eight umbilical cord sections, 14 atherosclerotic artery sections, and 10 gastrointestinal tract sections were examined by immunohistochemistry using polyclonal anti-CagA antibodies. Five atherosclerotic and 3 normal artery samples were also lysed in ice-cold lysis buffer containing protease inhibitors and were immunoprecipitated using the same antibodies. Anti-CagA antibodies reacted with cytoplasm and nuclei of smooth muscle cells in umbilical cord and atherosclerotic vessel sections, cytoplasm of fibroblasts-like cells in intimal atherosclerotic plaques, and the cell membranes of endothelial cells. Anti-CagA antibodies also specifically immunoprecipitated 2 high molecular weight antigens of 160 and 180 kDa from both normal and atherosclerotic artery lysates. Conclusions Anti-CagA antibodies cross-react with antigens of both normal and atherosclerotic blood vessels. We speculate that the binding of anti-CagA antibodies to those antigens in injured arteries could influence the progression of atherosclerosis in CagA-positive H pylori -infected patients.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Physiology (medical),Cardiology and Cardiovascular Medicine

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