Affiliation:
1. From the Institute of Internal Medicine and Geriatrics, University of Palermo (M.B., L.J.D.), Italy; and Hypertension Center, New York Presbyterian Hospital/Weill Medical College of Cornell University (O.B., L.M.R.).
Abstract
Abstract
—
— Previous studies by our group have identified ionic aspects of insulin resistance in hypertension, in which cellular responses to insulin were influenced by the basal intracellular ionic environment—the lower the cytosolic free magnesium (Mg
i
), the less Mg
i
increased following insulin stimulation. To investigate whether this ionic insulin resistance represents a more general abnormality of cellular responsiveness in hypertension, we studied Mg
i
responses to nonhormonal signals such as hyperglycemia (15 mmol/L) and used
31
P-nuclear magnetic resonance (NMR) spectroscopy to measure Mg
i
in erythrocytes from normal (NL, n=14) and hypertensive (HTN, n=12) subjects before and 30, 60, 120, and 180 minutes after in vitro glucose incubations. Basal Mg
i
levels were significantly lower in HTN subjects than in NL subjects (169±10 versus 205±8 μmol·L
−1
,
P
<0.01). In NL cells, hyperglycemia significantly lowered Mg
i
, from 205±8 μmol·L
−1
(basal, T=0) to 181±8, 162±6, 152±7, and 175±9 μmol·L
−1
(T=30, 60, 120, and 180, respectively;
P
<0.005 versus T=0 at all times). In HTN cells, maximal Mg
i
responses to hyperglycemia were blunted, from 169±10 μmol·L
−1
(basal, T=0) to 170±11, 179±12, 181±14, and 173±15 μmol·L
−1
(T=30, 60, 120, and 180, respectively;
P
=NS versus T=0 at all times). For all subjects, Mg
i
responses to hyperglycemia were closely related to basal Mg
i
levels: the higher the Mg
i
, the greater the response (n=26,
r
=0.620,
P
<0.001). Thus, (1) erythrocytes from hypertensive vis-à-vis normotensive subjects are resistant to the ionic effects of extracellular hyperglycemia on Mg
i
levels, and (2) cellular ionic responses to glucose depend on the basal Mg
i
environment. Altogether, these data support a role for altered extracellular glucose levels in regulating cellular magnesium metabolism and also suggest the importance of ionic factors in determining cellular responsiveness to nonhormonal as well as hormonal signals.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Cited by
14 articles.
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