Affiliation:
1. From the Center for Clinical Pharmacology (N.H.B.), the Department of Cardiology (M.B., T.T.N.), and the PET Center (F.H.), Aarhus University Hospital; the Center for Muscle Research, Copenhagen University (M.S.); and the Department of Pharmacology (M.J.M.), Aarhus University, Denmark.
Abstract
Background
Myocardial perfusion during adenosine-induced hyperemia is used both in clinical diagnosis of coronary heart disease and for scientific investigations of the myocardial microcirculation. The objective of this study was to clarify whether adenosine-induced hyperemia is dependent on endothelial NO production or is influenced by adrenergic mechanisms.
Methods and Results
In 12 healthy men, myocardial perfusion was measured with PET in 2 protocols performed in random order, each including 3 perfusion measurements. First, perfusion was measured at rest. Second, either saline or the NO synthase inhibitor
N
G
-nitro-
l
-arginine methyl ester (L-NAME, 4 mg/kg) was infused, and perfusion during adenosine-induced hyperemia was determined. Last, in both protocols, the α-receptor blocker phentolamine was infused, and perfusion during adenosine-induced hyperemia was determined again. Resting perfusion was similar in the 2 protocols (0.69±0.14 and 0.66±0.18 mL · min
−1
· g
−1
). L-NAME increased mean arterial blood pressure by 12±7 mm Hg (
P
<0.01) and reduced heart rate by 16±7 bpm (
P
<0.01). Adenosine-induced hyperemia (1.90±0.33 mL · min
−1
· g
−1
) was attenuated by L-NAME (1.50±0.55 mL · min
−1
· g
−1
,
P
<0.01). The addition of phentolamine had no effect on the adenosine-induced hyperemia (2.10±0.34 mL · min
−1
· g
−1
,
P
=NS). In the presence of L-NAME, however, when the adenosine response was attenuated, phentolamine was able to increase hyperemic perfusion (2.05±0.44 mL · min
−1
· g
−1
,
P
<0.05).
Conclusions
Inhibition of endogenous NO synthesis attenuates myocardial perfusion during adenosine-induced hyperemia, indicating that coronary vasodilation by adenosine is partly endothelium dependent. α-Adrenergic blockade has no effect on adenosine-induced hyperemia unless NO synthesis is inhibited.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Subject
Physiology (medical),Cardiology and Cardiovascular Medicine
Cited by
174 articles.
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