Mouse Model of Transplant Arteriosclerosis

Author:

Dietrich Hermann1,Hu Yanhua1,Zou Yiping1,Dirnhofer Stephan1,Kleindienst Roman1,Wick Georg1,Xu Qingbo1

Affiliation:

1. From the Institute for General and Experimental Pathology (H.D., Y.H., G.W.), University of Innsbruck Medical School; the Institute for Biomedical Aging Research (Y.Z., G.W., Q.X.), Austrian Academy of Sciences; and the Departments of Pathology (S.D.) and Internal Medicine (R.K.), University of Innsbruck Medical School, Innsbruck, Austria.

Abstract

Abstract —Transplant-accelerated arteriosclerosis in coronary arteries is the major limitation to long-term survival of patients with heart transplantation. The pathogenesis of this disease is not fully understood. Herein, we describe a simplified model of artery allografts in the mouse that allows us to take advantage of transgenic, knockout, or mutant animals. Common carotid arteries or aortic vessels were end-to-end allografted into carotid arteries between C57BL/6J and BALB/c mice. Neointimal lesions were observed as early as 2 weeks after surgery and had progressed at 4 and 6 weeks postoperatively. The lumen of grafted arteries was significantly narrowed due to neointima hyperplasia 4 weeks after transplantation. Using this model, we studied the role of intercellular adhesion molecule-1 (ICAM-1) in the development of transplant arteriosclerosis in ICAM-1–deficient mice. Neointimal lesions of artery grafts from ICAM-1 –/– C57BL/6J to BALB/c mice were reduced up to 60% compared with wild-type controls. MAC-1 (CD11b/18)-positive cells adhering to the surface of ICAM-1 –/– artery grafts were significantly less as identified by en face immunofluorescence, and these positive cells were more abundant in intimal lesions of artery grafts in wild-type mice. Furthermore, the major cell component of neointimal lesions 4 weeks after surgery was found to be α-actin–positive smooth muscle cells, which were significantly reduced in lesions of ICAM-1 –/– artery grafts. Thus, this model has been proven to be useful for understanding the mechanism of transplant arteriosclerosis. Our findings demonstrate that ICAM-1 is critical in the development of allograft arteriosclerosis via mediation of leukocyte adhesion to, and infiltration into, the vessel wall.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Cardiology and Cardiovascular Medicine

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