COVID-19 spread from Wuhan city to all provinces of China during the Spring Festival. Around tens of thousands of people have been infected. Some studies have demonstrated that the reason for the wider spread of COVID-19 was that SARS-CoV-2 presents a greater affinity for angiotensin-converting enzyme 2 (ACE2) than SARS-CoV. That means the number of ACE2 positive cells is related to the possibility of infection. The expression of ACE2 was mainly localized in the testis, heart, and kidney, but the expression landscape in testis is still unknown. Considering the critical roles of testicular cells for the transmission of genetic information between generations, more and more people are concerned about the effects of COVID-19 on the testis. In this study, we analyzed Single-cell RNA-seq datasets of the adult human testis. Results showed that ACE2 was expressed in both germ cells and somatic cells, among which Sertoli cells, Spermatogenic Stem cell, and Leydig cells are majority cluster. Moreover, we also found that the positive rate of ACE2 in testes of infertile men was higher than that of normal men, which indicates that SARS-CoV-2 may cause reproductive disorders through pathway activated by ACE2 and the men with reproductive disorder may more likely to be infected with SARS-CoV-2. Our results also showed that the expression level of ACE2 was related to the age of men, and the peak of positive rate was at 30 years old, which indicates young men are more likely to be infected by SARS-CoV-2. Together, this research provides a biological background of the potential route for infection of SARS-CoV-2 and may enable rapid deciphering male-related reproductive disorders induced by COVID-19.