Aim: To identify splatter and aerosol distribution resulting from dental aerosol generating procedures (AGPs) in the open plan clinic environment. A secondary aim is to explore the detailed time course of aerosol settling after an AGP. Methodology: Dental procedures were undertaken on a dental mannequin. Fluorescein dye was placed into the irrigation system of the high-speed air turbine handpiece for the first experimental design, and in the second, fluorescein dye was entered into the mannequin's mouth via artificial salivary ducts. Filter papers were placed at set distances around the open plan clinic environment to collect aerosol and splatter under various mitigating conditions including ventilation and aspiration flow rate. An 8-metre diameter rig was set up to investigate the effect of fallow time. Filter papers were analysed using imaging software and spectrofluorometric analysis. Results: The distribution of fluorescein contamination varied widely across the open plan clinic depending on the experimental conditions. Unmitigated (i.e. no suction) procedures have the potential to deposit contamination at large distances. Medium volume dental suction (159 L/min air) reduced contamination in the AGP bay by 53%, and in adjacent and distant bays/walkways by 81-83%. Low volume suction (40 L/min air) gave similar reductions. Cross-ventilation reduced contamination in adjacent and distant bays/walkways by 80-89%. In the most realistic model (dye in mouth with medium volume suction) the samples in distant bays (≥5 m head-to-head chair distance) either gave zero readings or very low readings (< 0.0016% of the fluorescein introduced into the system during the procedure). Almost all (99.99%) of the splatter detected was retained within the AGP bay/walkway. Time course experiments showed that after 10 minutes, very little additional contaminated aerosol settled. Conclusions: The cross-infection risk from conducting AGPs in an open plan clinic environment appears small, particularly when bays are ≥ 5 m apart. There is a major dilution effect from the instrument water spray and a substantial protective effect from using dental suction. The majority of aerosol settles in the first 10 minutes indicating that environmental cleaning may be appropriate after this time.