Elimination of False Positive Signals for von Willebrand Factor mRNA Detected by Reverse Transcription/PCR Methods Required an Oilgonucleotide Probe Distinct from the PCR Primer Sequences: An Example of a Common Avoidable Pitfall of PCR Technique

Author:

YAMAGUCHI Masahiko,KUMADA Kaoru,LEE Elaine Y.,SHAREFKIN John B.

Publisher

The Showa University Society

Reference20 articles.

1. 1) Wagner DD : Cell biology of Von Willebrand factor. Annu Rev Cell Biol 6 : 217-246 (1990)

2. 2) Sharefkin JB, Van Wart HE, Cruess DF, Albus RA and Levine EM : Adult human endothelial cell enzymatic harvesting : Estimates of efficiency and comparison of crude and partially purified bacterial collagenase preparations by replicate microwell culture and fibronectin degradation measured by enzyme-linked immunosorbent assay. J Vasc Surs 4: 567-577 (1986)

3. 3) Yamaguchi M, Du W, Gould KE, Dieffenbach CW, Cruess DF and Sharefkin JB: Effects of aspirin, dipyridamole, and dibutyryl cyclic adenosine monophosphate on platelet-derived growth factor A chain mRNA levels in human sap henous vein endothelial cells and smooth muscle cells. Surgery 110 : 377-384 (1991)

4. 4) Tsukada T, Tippens D, Gordon D, Ross Rand Gown AM : HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization. Am J Pathol 126: 51-60 (1987)

5. 5) Lowe T, Sharefkin JB, Yang S and Dieffenbach CW: A computer program for selection of oligonucleotide primers for polymerase chain reactions. Nucl Acids Res 18: 1757-1761 (1990)

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