Screening of drugs for Jiu Dan syndrome animal model

Abstract

Objective: To establish an experimental animal model that conforms to the traditional Chinese medicine (TCM) syndrome of Jiu Dan. Methods: Based on the historical discourses of TCM practitioners on the Jiu Dan syndrome, the study deeply analyzed the mechanisms behind the formation of Jiu Dan syndrome. Taking into full consideration the clinical diagnostic basis of the TCM syndrome animal model, which includes symptoms, etiology, treatment, related factors, and objective indicators, a high-fat diet combined with free access to ethanol was used to induce spleen and stomach deficiency in rats, followed by the induction of liver injury using carbon tetrachloride, aminogalactose, and α-naphthylisothiocyanate. By observing the general biological status and biochemical indicators of the rats, the study screened the three chemical modeling drugs, carbon tetrachloride, aminogalactose, and α-naphthylisothiocyanate, to determine the modeling factor combination that could simulate the formation mechanism of the Jiu Dan syndrome, and established an animal model that meets the characteristics of the Jiu Dan syndrome. Conclusion: Ethanol and α- naphthylisothiocyanate were selected as the modeling drugs for the TCM Jiu Dan syndrome animal model.