The chemistry of Crismer’s test for glucose in urine

Abstract

The study of what is going on in a chemical test is important, the more if the involved substance is glucose in urine. In the Crismer’s test the blood-red colour of safranin solution in alkaline medium is discharged if the glucose concentration is higher than 100 mg/dl. The reagent is not affected by uric acid or creatinine as is the case with the Fehling solution. The safranin test detects sugar in saliva when other reagents failed. Since the reaction mechanism of this test has not been advanced, we provide the electron flow, step by step.