Identification of bacterial pathogens isolated from smoked blue whiting fish (Micromesistius poutasou) from Odeomu market in Osun state Nigeria-Reference-Cited by-同舟云学术

Identification of bacterial pathogens isolated from smoked blue whiting fish (Micromesistius poutasou) from Odeomu market in Osun state Nigeria

Published:2023-08 Issue:2 Volume:3 Page:27-34
ISSN:2703-1322
Container-title:German Journal of Veterinary Research
language:en
Short-container-title:Ger. J. Vet. Res.

Author:

Aladejana Oluwatoyin M.,Zakka Mary,Ogunlade Ayodele O.,Thonda Oluwakemi A.

Abstract

The presence of microorganisms is one of the major factors affecting the quality of smoked fish sold in the open markets. Smoked blue whiting fish (Micromesistius poutasou), commonly called (Panla) sold in the Odeomu market in Osun State, were analyzed for microbial contaminants. Isolates were identified using conventional biochemical methods, and antibiotics susceptibility testing was carried out using the disc diffusion method. The total bacterial counts (TBC) results showed that the fish samples had high bacterial counts, ranging from 2.1×103 to 9.2×103 colony-forming units (CFU)/g. Bacteria isolated from the fish samples were: E. coli (45.46%), Enterobacter spp. (1.01%), Klebsiella spp. (6.06%), Proteus spp. (9.09%), Salmonella spp. (7.07%), Shigella spp. (19.19%), Bacillus spp. (4.04%) and Staphylococcus spp. (8.08%). The antibiotic sensitivity pattern of Gram-Negative bacteria indicated that all the isolates were resistant to more than three antibiotics. All E. coli isolates were resistant to augmentin and ceftazidime, 82.2% were resistant to cefuroxime, 17.7% to gentamicin, and 6.7% to ofloxacin. Screening of resistance genes showed that all six selected multiple antibiotic-resistant E. coli isolates tested harbored TEM gene, and two isolates (33.33%) harbored the aac (3)-II gene. None of the isolates harbored SHV, CTX-M, and qnr B genes. Our results showed that smoked blue whiting fish may pose a significant risk of spreading antibiotic-resistant bacteria that contain multiple antibiotic-resistance genes, highlighting a serious public health concern

Publisher

German Multidisciplinary Publishing Center

Subject

Genetics,Animal Science and Zoology

Reference37 articles.

1. Acharjee, M., Sultana, R., 2018. Consequences of γ-irradiation on the dissemination of microorganisms among sea fish in Bangladesh. Presented at the 31st BSM Annual Conference , Bangladesh Society of Microbiologist, Dhaka, Bangladesh.

2. AcharjeeIsrat, M., Islam, I., Noor, R., 2019. Effects of gamma irradiation on the propagation of microbial growth in commonly available meat in Bangladesh. International Food Research Journal 26, 1211–1218.

3. Adebayo-Tayo, Bukola.C., Onilude, Abiodun.A., Patrick, U.G., 2008. Mycofloral of Smoke-Dried Fishes Sold in Uyo, Eastern Nigeria. World Journal of Agricultural Sciences 4, 346–350.

4. Agu, K.C., Ukponmwan, I.O., Orji, M.U., Awah, N.S., Anaso, C.I., UdemezueInter, O.I., 2013. Prevalence of Pathogenic Bacteria in Smoked Fish sold in Major Retail Markets in Benin, Nigeria. International Journal of Applied Sciences and Engineering 1, 1–4.

5. Akinwumi, F.O., 2014. Effects of Smoking and Freezing on the Nutritive Value of African Mud Catfish, Clarias gariepinus Burchell, 1822. JAS 6. https://doi.org/10.5539/jas.v6n11p143.