The Characteristics of Growth of Bacilli Formed Fouling on Wooden Constructions

Abstract

Problem of biofilm formation have a great significance for environmental microbiological research. Biospheric microorganisms can form biofilm, that provide bacteria resistance to influence of different environmental factors. Some of the most common bacteria in biosphere are bacilli, among them there are film-forming strains. Bacillus spp. ia a well-known film forming microorganisms that colonize environmental objects. The biofilm fouling of underwater elements of small wooden constructions located on the Dnieper River near the city of the Dnipro (Ukraine) was studied. It was found that biofilms from surfaces of water constructions include bacilli. It is established that the mean values of CFU in samples from running and still water were (1.81±0.52)×108 and (1.83±0.53)×108 CFU / ml respectively per area of wooden sample approximately 1 cm2, while during the laboratory cultivation of the film, formed by these cultures on the plate, the number of cells was (4.90±0.93)×107 and (4.60±1.07)×107 CFU / ml per 1 cm2 of the well’s bottom, which was an approximate limit of the content of cells of the Bacillus spp. film per unit of area.