Affiliation:
1. Cairo University
2. El-Menoufia University
Abstract
In this study a simple, rapid and sensitive spectrophotometric method was developed for the determination of an antihistaminic drug chlorpheniramine maleate (CPM) in pure form, pharmaceutical preparations, spiked humane urine and spiked blood serum. This method was based on the formation of ion-pairs between the basic nitrogen of the CPM drug and four chromogenic reagents namely bromocresol purple (BCP), alizarine Red S (ARS), eriochrome cyanine R (ECR), and cresol red (CR). The extracted colored ion-pairs were measured spectrophotometrically at 390, 425, 503 and 408 nm for BCP, ARS, ECR and CR reagents, respectively. The different parameters that affect the color development between CPM drug and dyestuff reagents were extensively studied to determine the optimal conditions for the assay procedure. The reaction was studied as a function of the volume of reagents, nature of solvent, temperature, reaction time and stoichiometric ratio between the CPM drug and the reagents. Beer’s law was valid over the concentration ranges of 1-30, 1-10, 2-120 and 4-120 μg mL-1 of CPM drug using BCP, ARS, ECR and CR reagents, respectively. The Sandell sensitivity, molar absorptivity, limit of detection and limit of quantification were determined. Applications of the proposed procedure to the analysis of the pharmaceutical preparations, spiked humane urine and spiked blood serum gave reproducible and accurate results without any interference from excipients. The results obtained by the proposed method were in good agreement with those obtained by reported method. The method can be suggested for the routine analysis of the cited drug.
Publisher
AOA Academic Open Access Ltd.
Subject
Psychiatry and Mental health,Neuropsychology and Physiological Psychology
Reference27 articles.
1. M.M. Abdulbari, M.S. Ihsan, Simultaneous determination and validation of chlorpheniramine maleate, acetaminophen, phenylpropanolamine hydrochloride and caffeine in tablet dosage form by using reverse phase high performance liquid chromatography (RP-HPLC), Int. J. Pharm. Sci. 5 (2013).
2. A. Kaura et al., Spectrophotometric determination of chlorpheniramine maleate and phenylpropanolamine hydrochloride in dosage forms, Int. Curr. Pharm. J. 2(5) (2013) 97-100.
3. B. Rushikesh, N. Atul, S. Sanjay, Analytical method development and validation of phenylephrine hydrochloride, chlorpheniramine maleate, paracetamol and caffeine in bulk drug and tablet dosage form by RP-HPLC, Indoamerican. J. Pharm. Res. 3 (2013).
4. M. Maithani et al., Development and validation of a RP-HPLC method for the determinationof chlorpheniramine maleate and phenylephrine in pharmaceutical dosage form, Pharm. Glob. Int. J. Comp. Pharm. 5 (2010) 0976.
5. V.K. Redasani et al., Simultaneous determination of chlorpheniramine maleate, phenylephrine hydrochloride, paracetamol and caffeine in pharmaceutical preparation by RP-HPLC, Chem. Ind. Chem. Eng. Q. 19 (2013) 57−65.