The corrective effect of Au/Ag/Fe nanoparticles on oxidative stress in terms induced colon adenocarcinoma

Abstract

ABSTRACT. Studying biological markers of oxidative stress is crucial and relevant, as this process can trigger the transition from an organ's "normal state" to a "malignant lesion". The use of metal nanoparticles to correct oxidative stress manifestations in oncology is one of the most popular subjects of modern science and is actively being introduced into modern medicine. Practically all possible types of nanoparticles have found their use in solving different problems in oncology. Therefore, our study aimed to investigate the possibility of Au/Ag/Fe nanoparticles use for the correction of redox balance disorders of the antioxidant system under the conditions of induced colon adenocarcinoma. Materials and methods. The study was performed on 150 white male rats. Animals were kept in standard vivarium conditions. Division of experimental rats into groups: I - control intact group (40 individuals); II - experimental group (70 individuals) with N,N-dimethylhydrazine hydrochloride administration once a week for 30 weeks; ІІІ – an experimental group (40 animals) with daily intragastric administration of Au/Ag/Fe nanoparticles. Oxidative stress was assessed in colon tissue homogenate by changes in the concentrations of 8-iso-prostaglandin F2α (8-iso-PGF2α), TBC-active products, diene and triene conjugates, Schiff bases, as well as by the activities of catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, and reduced glutathione. Results. Due to this research, we established that Au/Ag/Fe nanoparticles use leads to a decrease in the concentration of 8-iso-prostaglandin F2α (8-iso-PGF2α), TBC-active substances, diene, triene conjugates, Schiff bases. As a result of the above, we observed a reduction of manifestations of oxidative stress and restoration of enzymes of the antioxidant system and its biological mediators of a non-enzymatic nature. The activity of catalase, superoxide dismutase, glutathione peroxidase, and the concentration of reduced glutathione was restored to control indicators. Conclusions. Therefore, the use of Au/Ag/Fe nanoparticles leads to the restoration of the balance of redox equilibrium, improving the antioxidant system with induced adenocarcinoma of the large intestine.