Abstract
Increasing concerns over the environmental impact of chemical pesticides have prompted the exploration of alternative, and eco-friendly solutions for controlling insect pests. The use of entomopathogenic fungus (EPF) as a biological control agent is of paramount importance. We aimed to investigate the effect of different concentrations of Metarhizium anisopliae (Metschnikoff) Sorokin on larval mortality and pupal formation, of sawfly larvae, a major pest of rose plants. Our study revealed a concentration-dependent effect of M. anisopliae on sawfly larvae. At 7 days after EPF exposure, the highest concentration (1 × 109 conidia/ml) led to a mortality rate of 65.0% and at the 11th day, the larval mortality was reached up to 82.5%. The highest EPF concentration resulted in a minimal pupal formation (7.5%). This study demonstrates the potential of M. anisopliae as a valuable biological control agent against sawfly larvae infestations in rose plants.