Age-related and Non-age—related Changes in 100 Surveyed Horse Brains

Abstract

Brains from 100 horses, aged 2-25 years, were systematically examined by histopathology at 46 different neuroanatomical sites. The horses were sourced from a slaughterhouse (group A, n = 57), from a kennel that collected dead animals, and from 2 diagnostic laboratories (group B, n = 43). All horses from group A and 26 horses from group B were examined by a veterinarian in the period before death. None of the horses were known to exhibit clinical signs suggestive of neurologic disease. Among the main changes identified were vacuolation in the neuropil ( n = 73), neurons ( n = 32), white matter ( n = 31), and focal perivascular lymphoid cell infiltrates ( n = 35). Spheroids were frequently seen ( n = 91), and 10 horses each had more than 10 spheroids in the cuneate or gracile nucleus. Statistically significant age-related changes noted included intraneuronal ( n = 97) and glial or extracellular lipofuscin deposition ( n = 41), hemosiderin deposition around blood vessels ( n = 60), and calcium depositions ( n = 24). One horse had low-grade nonsuppurative meningoencephalitis; Alzheimer type II cells were detected in the brains of 2 horses. Hyalinized vessel walls in the cerebellum were observed in 1 horse. It was concluded that some histopathologic changes are a frequent feature in equine brains, which has implications for the pathologists involved in equine neurology and disease surveillance.