COMPARATIVE IN VITRO ANALYSIS OF CYTOTOXICITY OF BODIPY-LUMINOPHERS AS POTENTIAL FLUORESCENT SENSORS FOR BIOLOGICAL SYSTEMS

Abstract

Boron dipyrrin (BODIPY) based luminophores are of considerable interest as promising components of optically active molecular devices for sensing, visualization, and theranostics of biological systems. However, the toxic effect of boron dipyrrin based luminophores of various structure have not been systematically studied until now. In this regard, the work shows the spectral characteristics of the main groups of BODIPY luminophores, which differ in the nature of substituents in the structure of the dipyrrine ligand, as well as in the length of the aromatic system, which can be used to solve practical problems of molecular sensorics of biosystems. The cytotoxicity of the compounds was evaluated. In the study within the framework of an integrated approach, two groups of methods were used to study the effect of the compounds under study at the cellular level: assessment of the cytotoxic effect on the morphological characteristics of blood cells using phase contrast microscopy with detection of altered cells and the intensity of free radical processes of cell membrane degradation. The results of the study of changes in the antioxidant system of blood samples during incubation with all dyes did not reveal significant differences. do not show cytotoxic effect. It was found that BODIPY has a potential membrane-protective effect, which was shown in this study for the first time.