MODIFICATION OF NANOCRYSTALLINE SILICON BY POLYMERS FOR BIOMEDICAL APPLICATIONS
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Published:2019-08-31
Issue:9
Volume:62
Page:86-96
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ISSN:2500-3070
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Container-title:IZVESTIYA VYSSHIKH UCHEBNYKH ZAVEDENII KHIMIYA KHIMICHESKAYA TEKHNOLOGIYA
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language:
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Short-container-title:IVKKT
Author:
Sharonova Nina V.,Ischenko Anatoly A.,Yagudaeva Elena Yu.,Sizova Svetlana V.,Smirnova Evgeniya V.,Ermakova Anna Yu.,Sviridov Alexander P.,Zubov Vitaly P.
Abstract
In this paper, it is proposed to use polymer-modified composite materials based on nanocrystalline silicon (nc-Si) as an alternative to organic fluorescent quantum dots traditionally used in medicine. A distinctive feature of nc-Si is a high absorption coefficient in the near UV and blue-violet range and the ability to transmit light in the visible region of the spectrum. The main advantage of silicon-based nanoparticles for in vivo use is their biodegradability and the absence of toxic properties. For hydrophilization of silicon nanoparticles, their surface was modified by amphiphilic biocompatible polymers: polyvinylpyrrolidone, a copolymer of maleic anhydride and 1-octadecene, cremophore, which is a polyoxyethylene derivative of hydrogenated castor oil. Silicon nanoparticles (nc-Si) with an average diameter of 4.5 nm, synthesized by annealing of SiO at 1150 °C, and functionalized with 1-octadecene photoluminescent in the red-infrared spectral region were used. The presence of the polymer shell on the surface of the nanoparticles was confirmed by FTIR spectroscopy. The sedimentation and aggregative stability of the particles in water were analyzed. It is shown that after the nc-Si polymer modification, the photoluminescent properties of nanoparticles are retained although the photoluminesce maxima were shifted to the blue region. Colorimetric MTT-assay of the cytotoxicity of the nanoparticles modified with polymers to monoclonal cells of human erythroleukemia K562 showed no toxicity for cells in culture at a particle concentration of up to 50 μg/ml. Subcellular localization of silicon nanoparticles into the human cervical carcinoma cell line HeLa was shown by means of fluorescence microscopy. The obtained polymer-modified nc-Si particles can be recommended for the purposes of bioimaging in in vitro and in vivo applications.
Publisher
Ivanovo State University of Chemistry and Technology
Subject
General Chemical Engineering,General Chemistry
Cited by
3 articles.
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