DETERMINATION OF CHAMAZULENE BY REVERSED PHASE HPLC

Abstract

A method for the determination of hamazulene in essential oils using reverse-phase high-performance liquid chromatography with diode-matrix detection has been developed. The possibility of qualitative determination of hamazulene from the electronic absorption spectra recorded in the detector cell and quantitative determination of this substance during detection at several different wavelengths is shown. To obtain chromatograms, it is proposed to use C18 stationary phase with a mobile phase containing 20 vol. % water in acetonitrile. Samples for the study were prepared by dissolving essential oils in acetonitrile. The chromatographic determination of hamazulene is not hindered by the accompanying components of the studied essential oils of four plants: Matricaria chamomilla, Achillea millefolium, Tanacetum annuum and Artemisia absinthium; the concentration of hamazulene in them was 0.184, 0.311, 0.299 and 0.0025 mol/l, respectively. The wavelength of 603 nm provides the most selective detection of hamazulene along a complex electron-vibrational band for a solution in the mobile phase. When the detection wavelength changes from 603 to 350 and up to 280 nm, the sensitivity of hamazulene detection increases by 15.5 and 187 times. The chromatographic method has a linear response of the detector despite the known features of the electronic absorption spectra of azulene derivatives. It was found that when a blue-colored spot of hamazulene is isolated from TLC plates, other nonpolar components of oils that are detected when recording a chromatogram under HPLC conditions at a detector wavelength of 210 nm also enter the extract. It was also found that when recording chromatograms at 200 nm, differentiation of oils is possible, since most of the components of these oils have at least one isolated double C=C bond in the structure, which allows detecting compounds at 190-200 nm. For citation: Deineka V.I., Salasina Ya.Yu. Determination of chamazulene by reversed phase HPLC. ChemChemTech [Izv. Vyssh. Uchebn. Zaved. Khim. Khim. Tekhnol.]. 2024. V. 67. N 6. P. 6-20. DOI: 10.6060/ivkkt.20246706.6989.