INVESTIGATION OF COMPONENTS OF HEXANE EXTRACT FROM TAMARIX HISPIDA BY METHOD OF GAS CHROMATOGRAPHY

Abstract

In this article, we determined the qualitative and quantitative component composition of the hexane extract, derived from plant material - the aerial part of the Tamarix hispida genus, the Tamarix family of Tamaricaceae, which grows in the southern region of the Republic of Kazakhstan (Almaty region). For this purpose, the previously dried and ground plant material (500 g) was extracted with hexane into the feed-reagent ratio (1:10) in a Soxhlet apparatus. The extract was concentrated under mild conditions (water bath temperature 40-45 °C) using a water-jet pump vacuum to concentrate. Analysis of the component composition of the hexane extract carried out using gas chromatography with a mass-selective detector under the following conditions: capillary column SHP-5MS used carrier gas helium with a flow rate of 1 ml / min at a temperature gradient from 40 °C to 250 °C and the injector volume - 0.2 μl. The materials were identified by mass spectra and retention times using the Wiley GC/MS library. The percentage of the components was calculated automatically from the areas of the peaks of the general chromatogram of the ions. As a result, 30 components were identified, of which 8 were acids and their esters, 5-alcohols, 4-ketones, 1-aldehyde, 1-phenol, 3-alkane, 3 halogen derivatives, 2-cyclic, 3-heterocyclic compounds. A significant content of β-carotene (23.73%), 2-cis-9-octadecinyl octaethanol (16.99%), propyl 3-octadecyloxyoleic acid (14.34%), octacosyltrifluoroacetate (8.60%), cis-11-octadecenoic acid (7.49%), heptacosan (6.01%), 2-bromocataladecan (5.24%), β-sitosterol (4.49%), respectively. Halogen-containing biologically active compounds were found for the first time in Tamarix genus. In addition, some amount of the nitrogen-containing compounds has been identified, which also were not previously found in lipophilic extracts of Tamarix.Forcitation:Ikhsanov Y.S., Abilov Z.A., Sultanova N.A., Choudhary M.I. Investigation of components of hexane extract from tamarix hispida by method of gas chromatography. Izv. Vyssh. Uchebn. Zaved. Khim. Khim. Tekhnol. 2018. V. 61. N 6. P. 83-87