STATIC AND DYNAMIC SORPTION OF NUCLEIC ACIDS AND PROTEINS ON SURFACE OF SORBENTS MODIFIED WITH NANOLAYERS OF POLYMERS

Abstract

Sorption properties of composite silica sorbents modified with nanolayers of fluoropolymers; polyanilines and polyaramides containing aromatic nitrogen; fluorine; as well as donor and acceptor fragments; with respect to nucleic acids (DNA and RNA) and proteins differing in molecular weight and pI are considered. The use of the investigated sorbents in the sample preparation for molecular diagnostics (in particular; in the PCR analysis) not only provides a one-step isolation of nucleic acids; but also allows the isolation and simultaneous purification of protein compounds from the impurities presented in the initial mixture. For the first time; the properties of these materials are compared in the static sorption regime using the compact spin-columns and in the regime of dynamic sorption by the method of spectral-correlation interferometry. The effect of the chemical composition; morphology; and surface charge of these polymer coatings on their sorption properties was studied. Possible mechanisms of sorption of biopolymers on the investigated sorbents are discussed. The use of the developed approaches to the analysis of properties of the sorbents as well as the obtained data open new possibilities for the synthesis of composite sorbents with specific properties. PANI and polyaramides were shown to demonstrate the similar sorption properties when interacting with nucleic acids; but they differ in a various extent in the retention of various proteins. In a neutral aqueous medium (optimal for separation of biopolymers) polyaramides; although did not retain DNA; had a weaker affinity to proteins as compared to PANI. Since the recovery of DNA passed through the PANI-coated silica was the maximal among the particulate adsorbents; the PANI-modified composites were preferred as the carriers for the single-step isolation of nucleic acids from complex biological mixtures. The article cites the results of systematic studies of the authors in the development of sorbents for one-step separation and isolation of biopolymers from complex biological mixtures.Forcitation:Liaw D.-J.; Zybin D.I.; Prostyakova A.I.; Yagudaeva E.Yu.; Vikhrov A.A.; Ishchenko A.A.; Zubov V.P.; Kapustin D.V. Static and dynamic sorption of nucleic acids and proteins on surface of sorbents modified with nanolayers of polymers. Izv. Vyssh. Uchebn. Zaved. Khim. Khim. Tekhnol. 2018. V. 61. N 1. P. 4-22