Multisite EGFR phosphorylation is regulated by adaptor protein abundances and dimer lifetimes-Reference-Cited by-同舟云学术

Multisite EGFR phosphorylation is regulated by adaptor protein abundances and dimer lifetimes

Published:2020-03-19 Issue:7 Volume:31 Page:695-708
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Salazar-Cavazos Emanuel¹²,Nitta Carolina Franco¹²,Mitra Eshan D.³,Wilson Bridget S.¹²,Lidke Keith A.²⁴,Hlavacek William S.²³,Lidke Diane S.¹²

Affiliation:

1. Department of Pathology

2. Comprehensive Cancer Center, and

3. Theoretical Biology and Biophysics Group, Theoretical Division, Los Alamos National Laboratory, Los Alamos, NM 87545

4. Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131

Abstract

Using a modified single-molecule pull-down (SiMPull) approach, the first direct detection of activation-dependent multisite phosphorylation on intact EGFR is provided. Integrating SiMPull data with rule-based modeling revealed roles for receptor dimerization dynamics and adaptor protein concentrations in directing EGFR signaling.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

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