Affiliation:
1. Department of Microbiology and Immunology, University of California–San Francisco, San Francisco, CA 94143-2200
Abstract
Candida albicans, the major human fungal pathogen, undergoes a reversible morphological transition from blastospores (round budding cells) to filaments (elongated cells attached end-to-end). This transition, which is induced upon exposure of C. albicans cells to a number of host conditions, including serum and body temperature (37°C), is required for virulence. Using whole-genome DNA microarray analysis, we describe 61 genes that are significantly induced (≥2-fold) during the blastospore to filament transition that takes place in response to exposure to serum and 37°C. We next show that approximately half of these genes are transcriptionally repressed in the blastospore state by three transcriptional repressors, Rfg1, Nrg1, and Tup1. We conclude that the relief of this transcriptional repression plays a key role in bringing the C. albicans filamentous growth program into play, and we describe the framework of this transcriptional circuit.
Publisher
American Society for Cell Biology (ASCB)
Subject
Cell Biology,Molecular Biology
Reference65 articles.
1. Arnaud, M. B., Costanzo, M. C., Skrzypek, G. B., Binkley, G., Lane, C., Miyasato, S. R., Sherlock, G. (2005). TheCandidaGenome Database (CGD), a community resource forCandida albicansgene and protein information.Nucleic Acids Res.33, D358-D363.
2. Bailey, D. A., Feldmann, P. J., Bovey, M., Gow, N. A., and Brown, A. J. (1996). TheCandida albicansHYR1 gene, which is activated in response to hyphal development, belongs to a gene family encoding yeast cell wall proteins.J. Bacteriol.178, 5353-5360.
3. Bennett, R. J., Uhl, M. A., Miller, M. G., and Johnson, A. D. (2003). Identification and characterization of aCandida albicansmating pheromone.Mol. Cell. Biol.23, 8189-8201.
4. Birse, C. E., Irwin, M. Y., Fonzi, W. A., and Sypherd, P. S. (1993). Cloning and characterization of ECE1, a gene expressed in association with cell elongation of the dimorphic pathogenCandida albicans.Infect. Immun.61, 3648-3655.
5. Bramley, T. A., Menzies, G. S., Williams, R. J., Kinsman, O. S., and Adams, D. J. (1991). Binding sites for LH inCandida albicans: comparison with the mammalian corpus luteum LH receptor.J. Endocrinol.130, 177-190.
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