Monitoring global changes in chromatin compaction states upon localized DNA damage with tools of fluorescence anisotropy-Reference-Cited by-同舟云学术

Monitoring global changes in chromatin compaction states upon localized DNA damage with tools of fluorescence anisotropy

Published:2020-06-15 Issue:13 Volume:31 Page:1403-1410
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Kesavan P. S.¹,Bohra Darshika¹,Roy Sitara¹,Mazumder Aprotim¹

Affiliation:

1. TIFR Centre for Interdisciplinary Sciences, Tata Institute of Fundamental Research Hyderabad, Hyderabad 500046, Telangana, India

Abstract

Fluorescence anisotropy imaging is used to measure the changes in compaction of undamaged chromatin upon induction of clustered double-strand breaks. Live-cell dynamics is combined with subsequent immunofluorescence. PCNA is recruited immediately at the site of damage, while forming foci of repair away from the site at later time points in regions of open chromatin.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

Reference41 articles.

1. DNA double-strand breaks promote methylation of histone H3 on lysine 9 and transient formation of repressive chromatin

2. Chromatin Structure Exhibits Spatio-Temporal Heterogeneity within the Cell Nucleus

3. ATM Alters the Otherwise Robust Chromatin Mobility at Sites of DNA Double-Strand Breaks (DSBs) in Human Cells

4. Spatio-Temporal Plasticity in Chromatin Organization in Mouse Cell Differentiation and during Drosophila Embryogenesis

5. Quantitative Live Imaging of Endogenous DNA Replication in Mammalian Cells

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