Optical flow analysis reveals that Kinesin-mediated advection impacts the orientation of microtubules in theDrosophilaoocyte-Reference-Cited by-同舟云学术

Optical flow analysis reveals that Kinesin-mediated advection impacts the orientation of microtubules in theDrosophilaoocyte

Published:2020-06-01 Issue:12 Volume:31 Page:1246-1258
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Drechsler Maik¹²³,Lang Lukas F.⁴,Al-Khatib Layla¹,Dirks Hendrik⁵,Burger Martin⁶,Schönlieb Carola-Bibiane⁴,Palacios Isabel M.¹²

Affiliation:

1. School of Biological and Chemical Sciences, Queen Mary University of London, London E1 4NS, UK

2. Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, United Kingdom

3. Department of Zoology and Developmental Biology, University of Osnabrück, 49076 Osnabrück, Germany

4. Department of Applied Mathematics and Theoretical Physics, University of Cambridge, Cambridge CB3 0WA, United Kingdom

5. Institute for Computational and Applied Mathematics, University of Münster, 48149 Münster, Germany

6. Department of Mathematics, Friedrich-Alexander Universität Erlangen-Nürnberg, 91058 Erlangen, Germany

Abstract

Here we introduce an optical flow motion estimation approach to study microtubule (MT) orientation in the Drosophila oocyte, a cell displaying substantial cytoplasmic streaming. We show that MT polarity is affected by the regime of these flows and, furthermore, that the presence of flows is necessary for MTs to adopt their proper polarity.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

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