Fascin-induced actin protrusions are suppressed by dendritic networks in giant unilamellar vesicles

Abstract

By reconstituting minimal actin cytoskeletal systems inside giant vesicles, we show that dendritic and bundled actin networks compete to assemble actin networks. Encapsulation of fascin and Arp2/3 complex results in increased dendritic network aggregation and inhibited bundled actin-induced protrusions compared to encapsulating actin and fascin alone.