Distinct RNA polymerase transcripts direct the assembly of phase-separated DBC1 nuclear bodies in different cell lines-Reference-Cited by-同舟云学术

Distinct RNA polymerase transcripts direct the assembly of phase-separated DBC1 nuclear bodies in different cell lines

Published:2021-11-01 Issue:21 Volume:32 Page:
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Mannen Taro¹,Goto Masato¹,Yoshizawa Takuya¹,Yamashita Akio²,Hirose Tetsuro³,Hayano Toshiya¹

Affiliation:

1. College of Life Sciences, Ritsumeikan University, Kusatsu 525-8577, Japan

2. Graduate School of Medicine, University of the Ryukyus, Nishihara-cho 903-0215, Japan

3. Graduate School of Frontier Biosciences, Osaka University, Suita 565-0871, Japan

Abstract

Distinct RNA polymerase transcripts direct the assembly of phase-separated DBC1 nuclear bodies in different cell lines. We show two types of RNase-sensitive DBC1-contaning nuclear bodies (DNB and SNB) that exhibit phase-separated features and are constructed distinct RNA polymerase transcripts. Detailed domain dissection revealed that the RNA-binding domains of HNRNPL, as well as the intrinsically disordered proline-rich domain, can play significant roles in DNB formation and in vitro droplet formation.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

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