Quantitative and time-resolved monitoring of organelle and protein delivery to the lysosome with a tandem fluorescent Halo-GFP reporter-Reference-Cited by-同舟云学术

Quantitative and time-resolved monitoring of organelle and protein delivery to the lysosome with a tandem fluorescent Halo-GFP reporter

Published:2022-05-15 Issue:6 Volume:33 Page:
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Rudinskiy M.¹²³,Bergmann T. J.¹,Molinari M.¹³

Affiliation:

1. Università della Svizzera italiana, CH-6900 Lugano, Switzerland

2. Institute for Research in Biomedicine, CH-6500 Bellinzona, Switzerland

3. Department of Biology, Swiss Federal Institute of Technology, CH-8093 Zurich, Switzerland

Abstract

A tandem-fluorescent reporter consisting of a HaloTag-GFP chimera appended at the C- or at the N-terminus of select polypeptides to monitor protein and organelle delivery to the lysosomal district in a quantitative and time-resolved manner

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

Reference43 articles.

1. Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells.

2. ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy

3. Intrinsically Disordered Protein TEX264 Mediates ER-phagy

4. HaloTag Technology: A Versatile Platform for Biomedical Applications

5. ER ‐to‐lysosome‐associated degradation of proteasome‐resistant ATZ polymers occurs via receptor‐mediated vesicular transport

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