Serotonin 5-HT<sub>7</sub> receptor slows down the G<sub>s</sub> protein: a single molecule perspective-Reference-Cited by-同舟云学术

Serotonin 5-HT₇ receptor slows down the G_s protein: a single molecule perspective

Published:2023-08-01 Issue:9 Volume:34 Page:
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Petelák Aleš¹,Lambert Nevin A.²,Bondar Alexey³⁴^ORCID

Affiliation:

1. Laboratory of Structural Biology and Bioinformatics, Institute of Microbiology of the Czech Academy of Sciences, CZ-37333, Nové Hrady, Czech Republic

2. Department of Pharmacology and Toxicology, Augusta University, Augusta, GA 30912

3. Department of Molecular Biology and Genetics, Faculty of Science, University of South Bohemia, CZ - 37005, České Budějovice, Czech Republic

4. Laboratory of Microscopy and Histology, Institute of Entomology, Biology Centre of the Czech Academy of Sciences, CZ - 37005, České Budějovice, Czech Republic

Abstract

The 5-hydroxytryptamine receptor type 7 (5-HT7R) has been shown to exhibit noncanonical inverse coupling with its cognate Gs protein. Here, using single-molecule imaging, we show that the presence of 5-HT7R, particularly in the inactive state, dramatically reduces the diffusion rate of Gs.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

Reference20 articles.

1. Cell-substrate contacts illuminated by total internal reflection fluorescence.

2. The Expanded Biology of Serotonin

3. Components of the G s signaling cascade exhibit distinct changes in mobility and membrane domain localization upon β 2 ‐adrenergic receptor activation

4. Dissociated GαGTP and Gβγ Protein Subunits Are the Major Activated Form of Heterotrimeric Gi/o Proteins

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