A dedicated cytoplasmic container collects extrachromosomal DNA away from the mammalian nucleus-Reference-Cited by-同舟云学术

A dedicated cytoplasmic container collects extrachromosomal DNA away from the mammalian nucleus

Published:2023-10-01 Issue:11 Volume:34 Page:
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Schenkel Laura¹²^ORCID,Wang Xuan¹²,Le Nhung¹²,Burger Michael¹,Kroschewski Ruth¹^ORCID

Affiliation:

1. Institute of Biochemistry, Eidgenössische Technische Hochschule Zürich, Otto-Stern-Weg 3, 8093 Zürich, Switzerland

2. Molecular Life Science PhD Program, Life Science Zurich Graduate School, 8057 Zurich, Switzerland

Abstract

Mammalian cells express transfected plasmid DNA transiently, but the underlying mechanisms are not well known. Plasmid DNA was found to be captured in the cytoplasmic, (ER)Endoplasmic reticulum-enwrapped exclusome, which clusters both endogenous and exogenous extrachromosomal DNA. Exclusome-envelope formation corresponded to early but not late steps of nuclear-envelope formation. This work suggests that a cell-autonomous genome protection system recognizes, collects, and maintains extrachromosomal DNA in somatic mammalian cells.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

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