TheDrosophilaKinesin-like Protein KLP67A Is Essential for Mitotic and Male Meiotic Spindle Assembly-Reference-Cited by-同舟云学术

TheDrosophilaKinesin-like Protein KLP67A Is Essential for Mitotic and Male Meiotic Spindle Assembly

Published:2004-01 Issue:1 Volume:15 Page:121-131
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Gandhi Rita¹,Bonaccorsi Silvia²,Wentworth Diana¹,Doxsey Stephen³,Gatti Maurizio,Pereira Andrea²

Affiliation:

1. Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655

2. Istituto Pasteur-Fondazione Cenci Bolognetti and Istituto di Biologia e Patologia Molecolari del Consiglio Nazionale delle Ricerche, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma “La Sapienza”, P.le Aldo Moro 5, 00185 Rome, Italy

3. Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01655

Abstract

We have performed a mutational analysis together with RNA interference to determine the role of the kinesin-like protein KLP67A in Drosophila cell division. During both mitosis and male meiosis, Klp67A mutations cause an increase in MT length and disrupt discrete aspects of spindle assembly, as well as cytokinesis. Mutant cells exhibit greatly enlarged metaphase spindle as a result of excessive MT polymerization. The analysis of both living and fixed cells also shows perturbations in centrosome separation, chromosome segregation, and central spindle assembly. These data demonstrate that the MT plus end-directed motor KLP67A is essential for spindle assembly during mitosis and male meiosis and suggest that the regulation of MT plus-end polymerization is a key determinant of spindle architecture throughout cell division.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

Reference54 articles.

1. Adams, R.R., Tavares, A.A., Salzberg, A., Bellen, H.J., and Glover, D.M. (1998).pavarottiencodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis.Genes Dev.12, 1483–1494.

2. Basto, R., Gomes, R., and Karess, R.E. (2000). Rough Deal and Zw10 are required for the metaphase checkpoint inDrosophila.Nat. Cell Biol.2, 939–943.

3. Bonaccorsi, S., Giansanti, M.G., and Gatti, M. (1998). Spindle self-organization and cytokinesis during male meiosis inasterlessmutants ofDrosophila melanogaster.J. Cell Biol.142, 751–761.

4. Cassimeris, L. (1999). Accessory protein regulation of microtubule dynamics throughout the cell cycle.Curr. Opin. Cell Biol.11, 134–141.

5. Cenci, G., Bonaccorsi, S., Pisano, C., Verni, F., and Gatti, M. (1994). Chromatin and microtubule organization during premeiotic meiotic, and early postmeiotic stages ofDrosophila melanogasterspermatogenesis.J. Cell Sci.107, 3521–3534.

Cited by 72 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Maternal genetic variants in kinesin motor domains prematurely increase egg aneuploidy;2024-07-05

2. The cell cycle oscillator and spindle length set the speed of chromosome separation inDrosophilaembryos;2024-06-18

3. Analysis of the Selection Signal of the Tibetan Black Chicken Genome Based on Whole-Genome Sequencing;Genes;2023-08-24

4. Kinesin-8-specific loop-2 controls the dual activities of the motor domain according to tubulin protofilament shape;Nature Communications;2022-07-20

5. Biomechanics of chromosome alignment at the spindle midplane;Current Biology;2021-05