Cdc2 Kinase-dependent Disassembly of Endoplasmic Reticulum (ER) Exit Sites Inhibits ER-to-Golgi Vesicular Transport during Mitosis-Reference-Cited by-同舟云学术

Cdc2 Kinase-dependent Disassembly of Endoplasmic Reticulum (ER) Exit Sites Inhibits ER-to-Golgi Vesicular Transport during Mitosis

Published:2004-09 Issue:9 Volume:15 Page:4289-4298
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Kano Fumi¹,Tanaka Arowu R.¹,Yamauchi Shinobu¹,Kondo Hisao²³,Murata Masayuki¹

Affiliation:

1. Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo 153-8902, Japan

2. Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, United Kingdom

3. PRESTO, Japan Science and Technology Corporation, Saitama 332-0012, Japan

Abstract

We observed the disassembly of endoplasmic reticulum (ER) exit sites (ERES) by confocal microscopy during mitosis in Chinese hamster ovary (CHO) cells by using Yip1A fused to green fluorescence protein (GFP) as a transmembrane marker of ERES. Photobleaching experiments revealed that Yip1A-GFP, which was restricted to the ERES during interphase, diffused throughout the ER network during mitosis. Next, we reconstituted mitotic disassembly of Yip1A-GFP–labeled ERES in streptolysin O-permeabilized CHO cells by using mitotic L5178Y cytosol. Using the ERES disassembly assay and the anterograde transport assay of GFP-tagged VSVGts045, we demonstrated that the phosphorylation of p47 by Cdc2 kinase regulates the disassembly of ERES and results in the specific inhibition of ER-to-Golgi transport during mitosis.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

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