Iterative immunostaining combined with expansion microscopy and image processing reveals nanoscopic network organization of nuclear lamina

Abstract

Iterative indirect immunofluorescence staining (IT-IF) for expansion and structured illumination microscopy improves superresolution imaging of subnuclear nanostructures. The IT-IF increases antibody labeling density resulting in high intensity, while maintaining the signal-to-background ratio. Together with the robust denoising pipeline, IT-IF reveals the fine structural network of nuclear lamins.