Polo-like kinase 4 homodimerization and condensate formation regulate its own protein levels but are not required for centriole assembly

Abstract

Polo-like kinase 4 (Plk4) is the master regulator of centriole assembly. Here, the authors generate separation-of-function mutants to dissect the function of Plk4 homodimerization and condensate formation. It is shown that Plk4 dimerization and condensate formation are not required for centriole assembly but function to down-regulate Plk4 and suppress centriole overduplication.