A novel tool for the unbiased characterization of epithelial monolayer development in culture

Author:

Dawney Nicole S.1,Cammarota Christian2,Jia Qingyuan1,Shipley Alicia1,Glichowski Joseph A.2,Vasandani Muskaan1,Finegan Tara M.1,Bergstralh Dan T.123

Affiliation:

1. Department of Biology, University of Rochester, Rochester, NY 14627

2. Department of Physics & Astronomy, University of Rochester, Rochester, NY 14627

3. Department of Biomedical Genetics, University of Rochester Medical Center, Rochester, NY 14627

Abstract

A novel tool, ALAn (Automated Layer Analysis), is developed to classify the architecture of epithelial cell layers in three dimensions. The rearrangement of MDCK cells in the apical-basal axis is dependent on substrate availability, and the lack of substrate leads to disorganization.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

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