Transcription Profiling ofCandida albicansCells Undergoing the Yeast-to-Hyphal Transition

Author:

Nantel André1,Dignard Daniel1,Bachewich Catherine1,Harcus Doreen1,Marcil Anne1,Bouin Anne-Pascale1,Sensen Christoph W.2,Hogues Hervé1,van het Hoog Marco1,Gordon Paul2,Rigby Tracey1,Benoit François1,Tessier Daniel C.1,Thomas David Y.1,Whiteway Malcolm1

Affiliation:

1. Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada H4P 2R2; and

2. Institute for Marine Bioscience, National Research Council of Canada, Halifax, Nova Scotia, Canada B3H 3Z1

Abstract

The ability of the pathogenic fungus Candida albicans to switch from a yeast to a hyphal morphology in response to external signals is implicated in its pathogenicity. We used glass DNA microarrays to investigate the transcription profiles of 6333 predicted ORFs in cells undergoing this transition and their responses to changes in temperature and culture medium. We have identified several genes whose transcriptional profiles are similar to those of known virulence factors that are modulated by the switch to hyphal growth caused by addition of serum and a 37°C growth temperature. Time course analysis of this transition identified transcripts that are induced before germ tube initiation and shut off later in the developmental process. A strain deleted for the Efg1p and Cph1p transcription factors is defective in hyphae formation, and its response to serum and increased temperature is almost identical to the response of a wild-type strain grown at 37°C in the absence of serum. Thus Efg1p and Cph1p are needed for the activation of the transcriptional program that is induced by the presence of serum.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

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