Measuring nucleus mechanics within a living multicellular organism: Physical decoupling and attenuated recovery rate are physiological protective mechanisms of the cell nucleus under high mechanical load-Reference-Cited by-同舟云学术

Measuring nucleus mechanics within a living multicellular organism: Physical decoupling and attenuated recovery rate are physiological protective mechanisms of the cell nucleus under high mechanical load

Published:2020-08-01 Issue:17 Volume:31 Page:1943-1950
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Zuela-Sopilniak Noam¹,Bar-Sela Daniel²,Charar Chayki¹,Wintner Oren²,Gruenbaum Yosef¹,Buxboim Amnon²³

Affiliation:

1. Departments of Genetics, Hebrew University of Jerusalem, Jerusalem 9190401, Israel

2. Cell and Developmental Biology, The Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 9190401, Israel

3. Alexander Grass Center for Bioengineering, The Rachel and Selim Benin School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem 9190416, Israel

Abstract

A mechanical characterization of cell nuclei within living Caenorhabditis elegans nematodes reveals two protective mechanisms: (1) cytoskeletal decoupling of the nucleus at high load and (2) slow release rates of stored mechanical energy. Lamin knockdown softened the nucleus and attenuated deformation recovery, whereas aging increased nucleus stiffness.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

Reference47 articles.

1. Mechanics in human fibroblasts and progeria: Lamin A mutation E145K results in stiffening of nuclei

2. Nuclear mechanotransduction: sensing the force from within

3. A laminopathic mutation disrupting lamin filament assembly causes disease-like phenotypes inCaenorhabditis elegans

4. The Caenorhabditis elegans SUN protein UNC-84 interacts with lamin to transfer forces from the cytoplasm to the nucleoskeleton during nuclear migration

5. THE GENETICS OF CAENORHABDITIS ELEGANS

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