Increased numbers of nucleoli in a genome-wide RNAi screen reveal proteins that link the cell cycle to RNA polymerase I transcription

Abstract

The nucleolus is a dynamic nuclear condensate and site of ribosome biogenesis. Using wide-field fluorescence microscopy, we screened for proteins that when depleted cause an increase in nucleolar number. Our results uncovered an unexpected subset of proteins that link the nucleolus, cell cycle regulation, and RNA polymerase I transcription.