pHLARE: a new biosensor reveals decreased lysosome pH in cancer cells-Reference-Cited by-同舟云学术

pHLARE: a new biosensor reveals decreased lysosome pH in cancer cells

Published:2021-01-15 Issue:2 Volume:32 Page:131-142
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Webb Bradley A.¹,Aloisio Francesca M.¹,Charafeddine Rabab A.¹,Cook Jessica¹,Wittmann Torsten¹,Barber Diane L.¹

Affiliation:

1. Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94941

Abstract

Dyes for measuring lysosome pH (pHlys) cannot be propagated in cells for longitudinal or in vivo studies. We report a new genetically encoded ratiometric pHlys biosensor, pHLARE (pH Lysosomal Activity REporter), which we use to show decreased pHlys with inhibiting mTORC1 (mammalian target of rapamycin complex 1) activity and in cancer cells of different tissues and genetic signatures.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

Reference67 articles.

1. Tamoxifen inhibits acidification in cells independent of the estrogen receptor

2. The lysosome: from waste bag to potential therapeutic target

3. Lysosome Transport as a Function of Lysosome Diameter

4. Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons

5. Lysosomal alkalization and dysfunction in human fibroblasts with the Alzheimer’s disease-linked presenilin 1 A246E mutation can be reversed with cAMP

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