A quantitative and semiautomated method for determining misaligned and lagging chromosomes during mitosis-Reference-Cited by-同舟云学术

A quantitative and semiautomated method for determining misaligned and lagging chromosomes during mitosis

Published:2021-04-19 Issue:9 Volume:32 Page:880-891
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Gama Braga Luciano¹²³,Prifti Diogjena Katerina¹²³,Garand Chantal²,Saini Pawan Kumar⁴²³,Elowe Sabine²³⁵

Affiliation:

1. Programme en Biologie cellulaire et moléculaire, Faculté de Médicine Université Laval, Québec, QC G1V 0A6, Canada

2. Centre de recherche du Centre Hospitalier Universitaire (CHU) de Québec-Université Laval, Axe de réproduction, santé de la mère et de l’enfant, Québec, QC G1V 4G2, Canada

3. PROTEO-regroupement québécois de recherche sur la fonction, l’ingénierie et les applications des protéines, Québec, QC G1V 0A6, Canada

4. Programme en Médicine moléculaire, Faculté de Médicine Université Laval, Québec, QC G1V 0A6, Canada

5. Département de Pédiatire, Faculté de Médicine, Université Laval et le Centre de recherche sur le cancer de l’Université Laval, Québec, QC G1R 2J6, Canada

Abstract

This study uses Cartesian plane geometry to model mitotic cells and describe mathematical methods based on this to quantify chromosome misalignment and measure kinetochore position to metaphase plates. The method is adapted to count lagging kinetochores in anaphase and provide semiautomated, user-friendly ImageJ macros to facilitate the operations.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

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