CRISPR-Trap: a clean approach for the generation of gene knockouts and gene replacements in human cells-Reference-Cited by-同舟云学术

CRISPR-Trap: a clean approach for the generation of gene knockouts and gene replacements in human cells

Published:2018-01-15 Issue:2 Volume:29 Page:75-83
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Reber Stefan¹²,Mechtersheimer Jonas¹²,Nasif Sofia¹,Benitez Julio Aguila³,Colombo Martino¹²,Domanski Michal¹,Jutzi Daniel¹²,Hedlund Eva³,Ruepp Marc-David¹

Affiliation:

1. Department of Chemistry and Biochemistry, University of Bern, CH-3012 Bern, Switzerland

2. Graduate School for Cellular and Biomedical Sciences, University of Bern, CH-3012 Bern, Switzerland

3. Department of Neuroscience, Karolinska Institutet,171 77 Stockholm, Sweden

Abstract

Conventional nonhomologous end joining–based gene knockouts can lead to the production of C-terminally truncated proteins with potentially residual or dominant negative functions. Combining CRISPR/Cas9 with gene traps targeting the first intron (CRISPR-Trap), however, completely prevents the expression of the open reading frame, resulting in clean gene knockouts.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

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