INVESTIGATION CORRELATION BETWEEN THE ANTIOXIDANT, ANTIMICROBIAL, ANTI-FUNGI ACTIVITIES AND CONTENT OF PHENOLIC COMPOUNDS OF RASPBERRY LEAF (RUBUS IDAEUS L.) EXTRACTS

Abstract

Objective: The purpose of work was study the total content of some biologically active substances (BAS), determine antimicrobial, anti-fungi and antioxidant activities of obtained raspberry leaf extracts, and study a correlation analysis between the content of natural compounds and antimicrobial/antifungal and antioxidant activities. Material and Method: The quantity of phenolic compounds, catechins, flavonoids and hydroxycinnamic acids was determined by spectrophotometric method of analysis, whereas organic acids by alkalimetric method; antioxidant activity of obtained extracts was evaluated by potentiometric method, antimicrobial and anti-fungi wad determined by method of "well". Result and Discussion: Results demonstrates the highest amount of polyphenols, flavonoids, catechins and hydroxycinnamic acids were 1.85±0.02, 1.01±0.02, 0.33±0.01 and 0.24±0.005% in 60% ethanolic extract, respectively. The organic acids were dominated in aqueous extract (1.02±0.02%). The most potent antioxidant property possessed 60% extract of raspberry leaf. There is a high correlation between the content of polyphenols, catechins and antioxidant activity, in the case of inhibition of S. aureus, P. aeruginosa, B. subtillis depends on polyphenols, catechins and antioxidant activity, whereas fungi C. albicans significantly depends only on the content of hydroxycinnamic acids as well as E. coli is not depend on any BAS. These findings show the great potential in the development and creation of new medicines with antimicrobial, antioxidant and antifungal effects that are not inferior to, and even superior to, the effects of synthetic analogues.