INCREASING THE EFFICIENCY OF ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIALS BY FREEZING PRETREATMENT

Abstract

This paper describes the differences between the efficiency of enzymatic hydrolysis of selected lignocellulosic materials after pretreatment by cyclic freezing and thawing. It also discusses the analysis of the positive effect of alkaline conditions on pretreatment. The selected materials were Populus alba L., wheat straw from Triticum aestivum L. and Cannabis sativa. Three pretreatment methods were used to compare the efficiency of enzymatic decomposition of cellulose and cellulose accessibility. The best results show the wheat straw pre-treated at -20°C in the freezer with NaOH addition with a concentration of monosaccharides of 56.6 g.l-1 compared to initial hydrolysed material with a concentration of monosaccharides of 24.4 g.l-1. The results show better digestibility of grass compared to wood dendromass