THE STRUCTURE OF THE ANTERIOR CORNEA EPITHELIUM OF THE SUS SCROFA DOMESTICUS AT THE CRYOPHIXATION

Author:

Khomyakova Nataliya V.1ORCID,Koloskova Eleonora L.2ORCID,Sidorov Ivan I.2ORCID

Affiliation:

1. Private healthcare Institution the Hospital Russian Railways - Medicine of the Bryansk City, Bryansk

2. Central Scientific and Methodological Veterinary Laboratory, Suponevo

Abstract

The classic histological picture of the anterior corneal epithelium, known from the results of standard histological techniques and light microscopy of preparations of chemically fixed material, describes the presence of three cell layers: basal - germinative, middle and superficial. On preparations of cryofixed material, another homogeneous layer is identified above the cells of the third layer, which is usually identified as the precorneal tear film, that is, a layer of liquid. Analysis and comparison of known data allows us to identify a scientific problem, which is as follows. The structure of the homogeneous layer has not been previously studied, its structure is unknown, therefore, the identification of the homogeneous layer with the precorneal tear film is not justified. We assume that the histological picture of the anterior epithelium on preparations of chemically fixed material is incomplete, and the homogeneous layer is a structural component of the anterior epithelium. Purpose of the study: the structure of the anterior epithelium and homogeneous layer on preparations of cryofixed material. The material for the study was preparations of the eyes of the domestic pig Sus scrofa domesticus, the research method was light microscopy. As a result of the study, it was established for the first time that the homogeneous layer is a structural component of the anterior corneal epithelium. Between the homogeneous layer and the layer of flat cells of the anterior epithelium, another layer is determined in the form of a microscopically detectable thin strip. Taking into account the established fact, it should be concluded that the anterior epithelium of the cornea consists of five layers. The cells in the layers are in a compacted state, the degree of which increases from the basement membrane to the frontal surface of the epithelium, reaching a maximum at the level of the homogeneous layer. The cells of the surface layer are anucleate, filled with an oxyphilic mass, presumably keratin, which indicates the process of keratinization of epithelial cells. The results of the study confirmed the assumption that the histological picture on preparations of chemically fixed material is incomplete. The identification of a homogeneous layer with the precorneal tear film is unfounded.

Publisher

Reaviz Medical University

Subject

General Medicine

Reference23 articles.

1. Metz А. The Anatomy and Histology of the Human Eye. Philadelphia, 1868. URL: https://archive.org/details/anatomyhistology00metzuoft

2. Ehlers N. Morphology and histochemistry of the corneal epithelium of mammals. Cells Tissues Organs. 1970;75(2):161–198. DOI:10.1159/000143448

3. Bülow N, Ehlers N. Morphology and dopa reaction of cultivated corneal epithelial cells. Acta Ophthalmologica. 2009;46(4):749–56. DOI:10.1111/j.1755-3768.1968.tb02873.x

4. McTigue JW. The human cornea: a light and electron microscopic study of the normal cornea and its alterations in various dystrophies. Trans Am Ophthalmol Soc. 1967;65:591–660. URL: https://pubmed.ncbi.nlm.nih.gov/4170533

5. DelMonte DW, Kim T. Anatomy and physiology of the cornea. J Cataract Refract Surg. 2011;37(3):588–598. DOI:10.1016/j.jcrs.2010.12.037

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3