Customized modified therapies for ovarian cyst and persistent follicle followed with TAI in early postpartum dairy cows

Abstract

Background: Postpartum ovarian dysfunction (ovarian cyst and persistent follicle) has been an important issue. Finding effective hormonal treatments to improve reproductive performance in dairy cows has become a necessity. Aim: Improve reproductive performance and ovarian activity in postpartum cows with specific customized treatment for ovarian cyst and persistent follicle. Methods: The study included 48 cows at 14 days P.P, which received two dosages of 500 μg IM cloprostenol, 14 days apart as presynchronization protocol. Ultrasound ovarian scans 14 days after the last injection for four weeks. The cows were divided into three groups according to ovarian status: OC (n=14), PF (n=12) and NE (n=22). In the OC group, received 500 μg IM cloprostenol and 100 μg IM cystoriline, a second dose of cloprostenol 14 days later and a second dose of cystoriline 36 hr. later, and AI after 24 hr. (GnRH+PG/PG/GnRH). In the PF group, were fitted with PRID for 9 days; the same day, they received 100 μg cystoreline then 500 μg cloprostenol 7 days later, after PRID removal AI 56 hours later (PRID+GnRH/PG). In the NE group, the artificial insemination was implemented until 28 days depended on estrus detection. Results: The ovarian activity was greatly affected by the customized treatments, leading to enhanced follicular and luteal activity, particularly after the PGF2α injection. The OC and PF groups showed substantial estrus responses of 71.43% and 75.02% respectively, during AI time. While the NE group had an ovulation rate of 54.5% and a pregnancy rate of 31.8%, the treatment groups showed marked improvements in reproductive performance. The ovulation rates in the OC and PF groups were 71.43 and 75% and the pregnancy rates at the 1st TAI were 64.28 and 66.7%. Conclusion: Improving reproductive performance and minimizing the time to first service are possible advantages of early case-specific treatment for postpartum cows with ovarian cysts and persistent follicles.