The influence of Rhus coriaria seeds Extracts on the Genetic Resistance of Aspergillus amstelodami

Abstract

Introduction: This study aims to investigate the safe use of Rhus coriaria seed extracts (water and ethanol) genetically on fungi Aspergillus amstelodami because the extensive use of plant in many pharmaceutical and food fields. Materials and methods: In this study A1 (Wa1) strain of the fungus A.spergillus amstelodami was used in all genetic testing, Rhus coriaria seeds were obtained from the local markets and two types of extracts were prepared (water and ethanol extracts of Rhus coriaria seeds), two types of resistance mutants were isolated both spontaneous and induced by using mutation agent (nitrous acid). Results and Discussion: In this study 18 spontaneous resistance mutation were isolated in frequency 4.26×10-5 and 96 induced mutations were induced by nitrous acid in Frequency39.76×10-5at (MIC) 16 mg / ml of the ethanol extract, and 22 spontaneous mutations were isolate in frequency 4.59×10-5 and 91 nitrous acid induced mutation with an average recurrence 37.36×10-5at (MIC) 25 mg / ml of water extract. Conclusion: We conclude that presence of resistant ability in A. amstelodami toward Rhus coriaria water and ethanol seeds extracts. Therefore, we suggest further thorough studies to detect the activity to plant extract in order to be use in agricultural pest control.