Effect of vanadium on nitrate reductase activity in tomato leaves

Author:

Buczek J.

Abstract

The activity of nitrate reductase in cell-free extracts from tomato leaves is completely inhibited by 100 μM NaVO<sub>3</sub> or VOCl<sub>2</sub>. In experiments in vivo vanadium ions inhibit the activity of the enzyme in 50 to 60 per cent. Addition of l mM vanadium to the medium on which tomato seedlings are grown causes after 24 h almost complete inhibition of nitrate reductase activity in cell-free extracts of the enzyme. Inhibition with vanadium may be abolished in experiments <i>in vitro</i> if the extract is treated with a rather concentrated EDTA solution or Sephadex G-25. The data obtained in this study indicate that vanadium ions in relatively low concentrations inhibit the activity of nitrate reductase in tomato seedlings both <i>in vivo</i> and <i>in nitro</i>.

Publisher

Polish Botanical Society

Subject

Plant Science

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