Impact of Vitamin D on Expression of SIRT7 and CYP24A1 in Human Breast Cancer Cells

Abstract

Objectives: Impact of vitamin D (1, 25 dihydroxy vitamin D3) (calcitriol) in the regulation of different genes has been investigated in different cancers including breast cancer (BC). Material and Methods: In the current study, we analyzed the expression levels of the CYP24A1 and SIRT7 genes and their relationship with patients’ clinical data in BC using polymerase chain reaction (PCR). Afterward, we tend to analyze the effect of vitamin D on the expression of these genes in cell lines (MCF7 and MDAMB231) to find the regulatory role of vitamin D in BC. Results: Our results showed that the CYP24A1 and SIRT7 were increased by vitamin D treatment and CYP24A1 levels were related to tumors stages (p = 0.03) and up-regulation of CYP24A1, SIRT7 had the distinguish potential for breast cancer detection based on their receiver operative characteristic (ROC) curve results (0.77, 0.84, respectively). Conclusion: In summary, CYP24A1, SIRT7 may be used as a possible diagnostic biomarker in BC treatment.